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Forty-four (NIH, Bethesda, Doctor). The particular cell-surface expression of tetraspanin CD9 and integrins ��v��3 and ��v��5 had been assessed simply by fluorescence-activated mobile selecting (FACS) analysis. Tissue ended up detached along with A single mmol/L EDTA throughout PBS and rinsed inside blocking remedy (PBS together with either 3.5% bovine serum albumin as well as standard goat serum)

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