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5, 150 mM NaCl, ��OG 1% (w/v), at room temperature away from light. Reactions were stopped by adding an equivalent volume of SDS-PAGE loading buffer. After electrophoresis, gels were visualized with a UV transilluminator at 312 nm before coomassie blue staining. MTS-EMCA fluorescence appeared for the labeled proteins. Crystallization and Data Collection OprM crystals were grown by vapor di

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